“San Juan De Dios", Zaragoza

Procedure and results

Sample collection method: with a sterile swab moistened in Brain Heart Infusion (BHI) medium and rubbing the surface to be analyzed for 30 seconds.

The swab was cut with sterile scissors and introduced into the BHI medium, immediately transporting it to the laboratory where it was mixed with vortex for 30 seconds. Subsequently, 0.5 ml of medium has been inoculated on the surface of the culture media selected for the isolation and quantification of aerobic bacteria and fungi:

- Mueller-Hinton agar
- MacConkey Agar
- Blood Agar
- Sabouraud Agar

The samples have been incubated aerobically for 48 hours at 37oC reading after 24 and 48 hours.

Suspicious multi-resistant organisms have been subcultured in chromogenic media and/or processed on the BD Phoenix Analyzer to detect:

- MRSA
- Vancomycin resistant Enterococci (VRE)
- ESBL-producing Enterobacteriaceae
- Carbapenemase-producing Enterobacteriaceae (CPE)
- Carbapenem-resistant Pseudomonas aeruginosa

Results from study

Standard cleaning and disinfection methods showed accumulated effective elimination of 84% (481 CFUs/cm2 after patient discharge down to 77 CFUs/cm2) of the aerobic bacteria in the four patient rooms.

The results after disinfection with a UVD Robot showed a total absence of aerobic bacteria on all sampled locations (77 CFUs/cm2 after manual cleaning and disinfection down to 0 CFUs/cm2 ).

Consequently, environmental disinfection with the UVD Robot proved to be an effective disinfection tool. The UVD Robot attributed to complementary inactivation efficacy to the traditional manual cleaning and disinfection, showing potential in reducing the healthcare associated infections (HAIs) in the hospital.

The total eradication of the environmental contamination breaks the chain of infection by limiting the risk of transmission, thereby limiting the chances of environmentally transmitted pathogens causing HAIs.